ACQUITY UPLC H-Class PLUS System for Method Lifecycle Management 

Efficient and Fast Quaternary System

The ACQUITY UPLC H-Class PLUS Quaternary System remains our most flexible, high-throughput method development platform. The H-Class PLUS Quaternary System delivers increased gradient precision in a robust configuration.  Whether you need to quickly develop new methods or analyze large numbers of samples, the H-Class PLUS decreases the time to result while increasing the depth and quality of information received from sample analysis. 


Extra power when you need it

With UPLC resolution and speed, automated column selection for six independently controlled column temperature zones, and up to six mobile phase options via a solvent select valve, the ACQUITY UPLC H-Class PLUS paired with the ACQUITY QDa Mass Detector and peak tracking delivers the power you need to explore an infinite set of conditions, while ensuring rigorous method development and validation for Method Lifecycle Management (MLCM) stages 1-2.


ACQUITY UPLC I-Class PLUS System for Method Lifecycle Management 

Maximum Precision Binary System 

More than any other LC configuration, the ACQUITY I-Class PLUS pushes the limits of precision and quantitation with the lowest dispersion on the market.  As the ideal inlet for high-performance mass spectrometers, the ACQUITY I-Class PLUS is designed for scientists who require the highest speed, sensitivity, resolution, and gradient precision. For ultrasensitive trace level quantitation, this system should be paired with the Waters Xevo TQ-XS Mass Spectrometer.  


Tailored Data Analysis

MassLynx Security with TargetLynx for low level quantitation.

Made for high resolution MS, this fundamental platform to acquire, analyze, manage, and share mass spectrometry information for high-throughput targeted quantification and confirmation analyses is complemented with a full-range of automatic quality control checks for the regulated laboratory. 



The I-Class PLUS and H-Class PLUS Systems can leverage the same components to deliver the right performance.   




The ACQUITY UPLC Photodiode Array Detector (PDA)  provides a lower dispersion flow cell, broad wavelength with 2D and 3D capable operation, unprecedented trace analyte detection and quantitation using broad spectrum optics of uncompromising linearity. 



ACQUITY Qda Angled

ACQUITY QDa Mass Detector 

If high resolution methods aren’t needed, the ACQUITY QDa Mass Detector can be used as a mass confirmation and peak-tracking tool. It requires minimal user training and automatically applies critical mass data to the peaks in your chromatogram, ensuring your analytes are properly identified and detectable, and impurities aren’t obscured by coelutions.




Empower 3 Chromatography Data Software (CDS)

The pharmaceutical industry-leading CDS, Waters Empower software ensures data traceability and delivers a customizable 21 CFR 11 compliant-ready solution to securely analyze, process, and report data to meet your requirements. 




S-Matrix Fusion QbD with PeakTracker  

Fusion QbD’s deep integration with Empower lets you dramatically accelerate method development and improve method optimization by adding: 

  1. Fully automated and flexible Design of Experiments (DOE) 
  2. UV and MS Based Peak Tracking, a powerful new technology to automate, optimize, and simplify the use of PDA and MS spectra data in LC and LC-MS method development. PeakTracker uses 3D PDA and MS spectra data augmented with standard UV peak results to automatically track peaks, including auto-deconvolution of co-eluted peaks 
  3. Hyper-accurate retention-based Resolution Map Modeling, along with modeling individual peak resolutions and all other required method chromatographic performance requirements 
  4. Instant automated modeling of all study parameter effects, including linear, non-linear, and interaction effects, on all your critical performance characteristics 
  5. Regulatory accepted integration of QbD Robustness 





Column Standards

Waters Column Chemistries & Vials 

Not all columns and vials are created equal.  Each lot of Waters columns undergoes rigorous validation testing to ensure not only initial quality, but also batch-to-batch consistency.  Likewise, each lot of Waters Certified Vials are tested for impurities using LC/MS to prevent sample contamination.  The ACQUITY UPLC H-Class PLUS and ACQUITY I-Class PLUS Systems for Method Lifecycle Management can be purchased with a wide range of scalable, reproducible, selective column chemistries and vials suitable for your analyses. See the online Waters consumables catalog to help you choose the column chemistry and vials that will best meet your needs.